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KMID : 0370220100540040215
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Yakhak Hoeji
2010 Volume.54 No. 4 p.215 ~ p.225
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Anti-oxidant and Anti-aging Activities of Essential Oils of Pinus densiflora Needles and Twigs
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Kim Youn-Joung
Cho Bong-Jae
Ko Myung-Soo
Jung Jae-Min
Kim Hee-Rae
Song Ho-sun
Lee Ji-Yun
Sim Sang-Soo
Kim Chang-Jong
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Abstract
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To investigate effects of pine essential oil isolated by steam distillation from Pinus densiflora needles (PN) and twigs (PT) on anti-oxidant and anti-aging activities, the experiments were carried out to determine anti-oxidant and antiaging activities on DPPH radical scavenging activity, NBT/xanthine oxidase-superoxide scavenging activity, silica-induced intracellular and hydroxyl radical generation in RAW264.7 cells, hyaluronidase and elastase activities in vitro. Essential oil of PN and PT were contained 0.225 and 0.176 (w/v) %, respectively. PN was contained with 30 kinds of essential oil and its major constituent is -pinene (21.5%). Further PT was contained with 40 kinds of essential oil and its major constituent is -pinene (22.4%) in GC/MS assay. Other essential oils of PN were -pinene, camphene, myrcene, limonene, terpinolene, -terpineol, bornyl acetate, -copaene, caryophellene and humulene and PT were -pinene, camphene, phellandrene, limonene, terpinolene, -terpineol, bornyl acetate, -copaene, caryophellene and humulene. The essential oil of PT have more active than that of PN in anti-oxidant activity which has significant DPPH radical and superoxide scavenging activity, and significant inhibitory activities on silica-induced intracellular and hydroxyl radical generation, as well as, significantly inhibited elastase and hyaluronidase activities. Further, phellandrene, myrcene and -pinene have DPPH radical and superoxide scavenging activities, -pinene, terpinolene, myrcene and phellandrene inhibited silica-induced intracellular and hydroxyl radical generations. And also phellandrene and -pinene inhibited hyaluronidase and elastase activities. In conclusion, the essential oils isolated from PN and PT have anti-oxidant and anti-aging activities.
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KEYWORD
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pinus essential oil, superoxide, hydroxyl radical, hyaluronidase
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